Oscillations in the peroxidase-oxidase reaction: a comparison of different peroxidases

The nonlinear behavior of the peroxidase-oxidase reaction was studied using structurally different peroxidases. For the first time sustained oscillations with peroxidases other than horseradish peroxidase in a single-enzyme system were observed. All peroxidases that showed significant oxidase activi...

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Bibliographic Details
Published in:Biochimica et biophysica acta 1996-04, Vol.1289 (3), p.397-403
Main Authors: Kummer, Ursula, Valeur, Kirsten Rosendal, Baier, Gerold, Wegmann, Klaus, Olsen, Lars Folke
Format: Article
Language:English
Subjects:
Animals
Cattle
Chloride Peroxidase - metabolism
Chlorophenols
Coprinus - enzymology
Dichlorophenol
Glycine max - enzymology
Horseradish Peroxidase - metabolism
In Vitro Techniques
Kinetics
Lactoperoxidase - metabolism
Methylene Blue
Milk - enzymology
Mitosporic Fungi - enzymology
Oscillation
Oscillometry - instrumentation
Oxidoreductases - metabolism
Peroxidase
Peroxidase-oxidase reaction
Peroxidases - metabolism
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Summary:The nonlinear behavior of the peroxidase-oxidase reaction was studied using structurally different peroxidases. For the first time sustained oscillations with peroxidases other than horseradish peroxidase in a single-enzyme system were observed. All peroxidases that showed significant oxidase activity were able to generate sustained oscillations. When adjusting the overall reaction rate, either of the two modifiers 2,4-dichlorophenol or Methylene blue could be omitted from the reaction. Due to the observation of different enzyme intermediates when using different peroxidases, we conclude that the mechanisms responsible for oscillatory kinetics may vary from one peroxidase to the other.
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/0304-4165(95)00176-X