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Somatic embryogenesis and plantlet regeneration in mandarin ( Citrus reticulata Blanco)

Callus cultures were initiated from leaf, epicotyl, cotyledon and root segments of in vitro grown nucellar seedlings of Citrus reticulata Blanco ‘Local Sangtra’. Callus initiation was seen on cut ends of all the explants within 10 days of culture. The epicotyl segments were the best explant for call...

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Bibliographic Details
Published in:Scientia horticulturae 1995-09, Vol.63 (3), p.167-174
Main Authors: Gill, M.I.S., Singh, Zora, Dhillon, B.S., Gosal, S.S.
Format: Article
Language:English
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Summary:Callus cultures were initiated from leaf, epicotyl, cotyledon and root segments of in vitro grown nucellar seedlings of Citrus reticulata Blanco ‘Local Sangtra’. Callus initiation was seen on cut ends of all the explants within 10 days of culture. The epicotyl segments were the best explant for callus induction. Best callus induction occurred on MS medium containing NAA (10 mg l −1) and kinetin (0.5 mg l −1). MS medium supplemented with NAA (10 mg l −1), kinetin (1 mg l −1) and vitamins (10 × normal MS levels) were best for induction of somatic embryogenesis. The epicotyl-derived callus exhibited significantly higher (83.0%) mean somatic embryogenesis than calli derived from other explants. Most of the somatic embryos germinated like normal embryos, but some only produced shoots. The time taken for root initiation from separated shoots was 7–10 days. The number of roots per shoot and root length also varied with culture media. Plantlet survival in vitro was directly proportional to the number of roots produced per shoot. In vitro derived plantlets were successfully transplanted to soil.
ISSN:0304-4238
1879-1018
DOI:10.1016/0304-4238(95)00812-8