Yeast cells with high β-galactosidase activity and their immobilization

The batch growth conditions of K. lactis were optimized for high β-galactosidase content using a modified inorganic salt medium with 1% lactose at 30 °C and pH as an indicator for the harvest time. Cell β-galactosidase activity of up to 966 LAU (g cell) −1 was achieved when the cell wall was permeab...

Full description

Saved in:
Bibliographic Details
Published in:Journal of molecular catalysis 1984-01, Vol.26 (2), p.173-185
Main Authors: Vlach, D., Prenosil, J.E.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Enzyme engineering
Fundamental and applied biological sciences. Psychology
Immobilization of enzymes and other molecules
Immobilization techniques
Methods. Procedures. Technologies
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The batch growth conditions of K. lactis were optimized for high β-galactosidase content using a modified inorganic salt medium with 1% lactose at 30 °C and pH as an indicator for the harvest time. Cell β-galactosidase activity of up to 966 LAU (g cell) −1 was achieved when the cell wall was permeabilized by Triton. The cells in batch operation tended to utilize glucose when it accumulated rather than lactose. The immobilized cells should be therefore used in a continuous plug flow reactor. The experiments with a 50 ml column reactor filled by the cells immobilized in polyurethane foam showed a good activity with no leakage of cells and a half-life time of approximately 3 d.
ISSN:0304-5102
DOI:10.1016/0304-5102(84)85001-4