Protein extractions by supported liquid membrane with reversed micelles as carriers

A phenomenological model has been proposed to describe the extraction of the enzyme, alpha-chymotrypsin, between the bulk aqueous and reversed micellar phases using a stirred membrane cell (SMC). The strong dependence of transport kinetics at the interface and through the membrane on the surfactant...

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Bibliographic Details
Published in:Journal of membrane science 1995-04, Vol.100 (2), p.87-97
Main Authors: Shau-wei Tsai, Chah-Lin Wen, Jun-Lone Chen, Chung-Shing Wu
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Enzyme engineering
Enzyme extraction
Fundamental and applied biological sciences. Psychology
Improved methods for extraction and purification of enzymes
Methods. Procedures. Technologies
Reversed micelle
Supported liquid membrane
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Summary:A phenomenological model has been proposed to describe the extraction of the enzyme, alpha-chymotrypsin, between the bulk aqueous and reversed micellar phases using a stirred membrane cell (SMC). The strong dependence of transport kinetics at the interface and through the membrane on the surfactant concentration indicates that the former is the rate-determining step at low surfactant concentration, and the latter is that at high surfactant concentration, respectively. Measurements on the enzyme recovery in a supported liquid membrane (SLM) with reversed micelles as carriers demonstrated the feasibility of the present process for enzyme purification.
ISSN:0376-7388
1873-3123
DOI:10.1016/0376-7388(94)00260-6