RNAs of two viruses of the Nudaurelia beta family share little homology and have no terminal poly(A) tracts

cDNA‐RNA liquid hybridization analysis was used to compare the RNA sequence homology between two members of the Nudaureliaβ virus family, Trichoplusia ni virus (T.ni V) and Dasychira pudibunda virus (D.p V). Heterologous hybridization experiments demonstrated that these viruses shared little sequenc...

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Bibliographic Details
Published in:FEMS microbiology letters 1985, Vol.26 (1), p.41-43
Main Authors: King, L.A, Moore, N.F
Format: Article
Language:English
Subjects:
Biological and medical sciences
cDNA‐RNA hybridization
Dasychira
Dasychira pudibunda
Fundamental and applied biological sciences. Psychology
hybridization
insect viruses
Microbiology
Morphology, structure, chemical composition, physicochemical properties
Nudaurelia
Nudaureliaβ viruses
poly(A) tract
RNA
Trichoplusia ni
Virology
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Summary:cDNA‐RNA liquid hybridization analysis was used to compare the RNA sequence homology between two members of the Nudaureliaβ virus family, Trichoplusia ni virus (T.ni V) and Dasychira pudibunda virus (D.p V). Heterologous hybridization experiments demonstrated that these viruses shared little sequence homology. Using oligo(dT) chromatography and oligo(dT)12–18 as a primer for cDNA synthesis it was shown that neither T.ni V nor D.p V RNA genomes possess a poly(A) tract at the 3′ end.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1985.tb01562.x