Anti-relaxin and relaxin on prolactin and gonadotropin secretion in vitro from porcine pituitary cells

This study examines the possibility of a feedback interaction between gonadal relaxin and the pituitary by investigating the impact of exogenous relaxin and ablation of endogenous with relaxin anti-relaxin serum on pituitary hormone secretion in vitro. Three wells were assigned to treatments: 0, 100...

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Bibliographic Details
Published in:Animal reproduction science 1995, Vol.40 (3), p.239-247
Main Authors: Musah, A.I, Barsness, B.D, Schrank, G.D
Format: Article
Language:English
Subjects:
Adenohypophysial cells
Antirelaxin
cerdo
gonadotrophine
Gonadotropin
gonadotropinas
gonadotropins
hormonas pituitarias
hormone hypophysaire
Luteal function
Pituitary
pituitary hormones
porcin
Prolactin
prolactina
prolactine
Relaxin
relaxina
relaxine
swine
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Summary:This study examines the possibility of a feedback interaction between gonadal relaxin and the pituitary by investigating the impact of exogenous relaxin and ablation of endogenous with relaxin anti-relaxin serum on pituitary hormone secretion in vitro. Three wells were assigned to treatments: 0, 100 and 1000 ng ml −1 of relaxin, 1:100, 1:1000 and 1:10000 titer of anti-relaxin. Relaxin significantly enhanced prolactin (PRL) secretion ( P < 0.05) in long-term culture but had no effect on luteinizing hormone and follicle stimulating hormone secretion. Relaxin anti-serum stimulated a dose dependent increase ( P < 0.05) in gonadotropin secretion at 48, 72 and 96 h. Luteinizing hormone and follicle stimulating hormone increased two-fold in 48 h cultures in response to 1:100 anti-relaxin serum in comparison with untreated controls. Anti-relaxin serum at 1:100 completely suppressed PRL secretion after either 48, 72, and 96 h of culture. At 48 h all levels of anti-relaxin serum completely suppressed PRL secretion. These results indicate that endogenous relaxin may be involved at the adenohypophysial level in modulating gonadotropin and PRL release in the pig.
ISSN:0378-4320
1873-2232
DOI:10.1016/0378-4320(95)01426-Z