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Species and individual variations in cryoprotectant toxicities and freezing resistances of epididymal sperm from African antelope

The potential toxicities and cryoprotective properties of glycerol, ethylene glycol, propylene glycol and dimethyl sulfoxide were examined for the cryopreservation of epididymal sperm from three species of African antelope: blesbok, impala, and springbok. Glycerol was found to be the least toxic tre...

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Bibliographic Details
Published in:Animal reproduction science 1996-04, Vol.42 (1), p.527-535
Main Authors: Loskutoff, Naida M., Simmons, Heather A., Goulding, Michelle, Thompson, Graham, De Jongh, Tim, Simmons, Lee G.
Format: Article
Language:English
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Summary:The potential toxicities and cryoprotective properties of glycerol, ethylene glycol, propylene glycol and dimethyl sulfoxide were examined for the cryopreservation of epididymal sperm from three species of African antelope: blesbok, impala, and springbok. Glycerol was found to be the least toxic treatment overall when epididymal sperm were incubated at 30–32°C to promote uptake and metabolism of the cryoprotective agents. Nevertheless, post-thaw functional survival, as measured by overall sperm motility, was equally effective using glycerol and ethylene glycol for blesbok and impala sperm. Springbok sperm, on the other hand, survived cryopreservation with the greatest percentage motility post-thawing using dimethyl sulfoxide. The results provide important preliminary data for the development of genetic resource banks for African antelope.
ISSN:0378-4320
1873-2232
DOI:10.1016/0378-4320(96)01545-X