A simple method for freezing rabbit semen with successful results on fertility and prolificity

The experiments were carried out to evaluate a simple method of freezing rabbit spermatozoa. The effect of glucose, lactose or sucrose on post-thawing sperm quality was studied. The results showed that addition of sucrose improved motility and acrosomal integrity (57% of progressive motile spermatoz...

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Bibliographic Details
Published in:Animal reproduction science 1996-10, Vol.44 (3), p.195-201
Main Authors: Viudes-de-Castro, M.P., Vicente, J.S.
Format: Article
Language:English
Subjects:
ALMACENAMIENTO EN FRIO
COLD STORAGE
CONEJO (ORYCTOLAGUS)
CONGELACION
CONGELATION
CONSERVACION DEL SEMEN
CONSERVATION DU SPERME
FERTILIDAD
FERTILITE
FERTILITY
FREEZING
LAPIN (ORYCTOLAGUS)
PERFORMANCE DE REPRODUCTION
Prolificity
Rabbit
RABBITS
REPRODUCTIVE PERFORMANCE
REPRODUCTIVIDAD
Semen cold storage
SEMEN PRESERVATION
STOCKAGE AU FROID
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Summary:The experiments were carried out to evaluate a simple method of freezing rabbit spermatozoa. The effect of glucose, lactose or sucrose on post-thawing sperm quality was studied. The results showed that addition of sucrose improved motility and acrosomal integrity (57% of progressive motile spermatozoa vs 43% and 42%, and 68% of normal acrosome vs 56 and 57%, sucrose, lactose and glucose, respectively). Then, a Tris-citrate medium with 0.05 M sucrose and 1.75 M dimethylsulphoxide (DMSO) was used to analyse the effect of frozen semen on the reproductive performance of does. Insemination of does with fresh and frozen semen showed no differences in fertility rate and prolificity between fresh and frozen semen (80% and 8.1 young rabbits, respectively).
ISSN:0378-4320
1873-2232
DOI:10.1016/0378-4320(96)01550-3