Determination of citalopram, amitriptyline and clomipramine in plasma by reversed-phase high-performance liquid chromatography

The determination of citalopram, amitriptyline, clomipramine and their desmethyl metabolites after alkaline diethyl ether extraction from plasma is achieved by high-performance liquid chromatography using two internal standards and μBondapak C 18 as stationary phase. Elution is carried out isocratic...

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Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1985-01, Vol.338, p.171-178
Main Authors: Rop, Pok Phak, Viala, A., Durand, A., Conquy, T.
Format: Article
Language:English
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Summary:The determination of citalopram, amitriptyline, clomipramine and their desmethyl metabolites after alkaline diethyl ether extraction from plasma is achieved by high-performance liquid chromatography using two internal standards and μBondapak C 18 as stationary phase. Elution is carried out isocratically at 0.5 or 1 ml/min with mixture of acetonitrile- potassium dihydrogen phosphate-distilled water (45:50:5). Detection is monitored by absorption at 254 nm. The detection limit is less than 5 ng/ml for each compound. The coefficients of variation are between 1.3% and 9.4% for 8-360 ng/ml. Interference from 22 possible co-medications is discussed. The technique can be used for therapeutic monitoring of these antidepressants as well as in analytical toxicology.
ISSN:0378-4347
DOI:10.1016/0378-4347(85)80081-5