Characterization and quantification of paf-acether (platelet-activating factor) as a heptafluorobutyrate derivative of 1-O-alkyl-2-acetyl- sn-glycerol by capillary column gas chromatography with electron-capture detection

A gas chromatographic method with a glass capillary column and electron-capture detection is proposed for the characterization and quantification of 1-O-hexadecyl-2-O-acetyl- sn-glycero-3-phosphocholine (PAF C 16) using the corresponding 1-O-hexadecyl-2-O-acetyl- sn-glycero-3-heptafluorobutyrate der...

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Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1987, Vol.423, p.23-31
Main Authors: Bossant, M.J., Farinotti, R., Mencia-Huerta, J.M., Benveniste, J., Mahuzier, G.
Format: Article
Language:English
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Summary:A gas chromatographic method with a glass capillary column and electron-capture detection is proposed for the characterization and quantification of 1-O-hexadecyl-2-O-acetyl- sn-glycero-3-phosphocholine (PAF C 16) using the corresponding 1-O-hexadecyl-2-O-acetyl- sn-glycero-3-heptafluorobutyrate derivative and, as an internal standard, 1-O-octadecyl-2-O-methyl- sn-glycero-3-phosphocholine. The reproducibility was approximately 5% and amounts as low as 20 pg could be measured. The method was specific and allowed the quantification of PAF C 16 in supernatants from stimulated human polymorphonuclear neutrophils.
ISSN:0378-4347
DOI:10.1016/0378-4347(87)80324-9