Determination of 3-(5-cyclopropyl-1,2,4-oxadiazol-3-yl)-5-(1-methylethyl)imidazo[1,5- a]quinoxalin-4(5 H)-one in serum by high-performance liquid chromatography

A high-performance liquid chromatographic assay with solid-phase extraction (SPE) for the rapid and sensitive quantitation of 3-(5-cyclopropyl-1,2,4-oxadiazol-3-yl)-5-(1-methylethyl)imidazo[1,5- a]quinoxalin-4(5 H)-one (I, U-78875) in serum is described. The validation results indicated that the pre...

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Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1991-02, Vol.563 (2), p.427-434
Main Author: Zhong, W.Z.
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacology. Drug treatments
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Summary:A high-performance liquid chromatographic assay with solid-phase extraction (SPE) for the rapid and sensitive quantitation of 3-(5-cyclopropyl-1,2,4-oxadiazol-3-yl)-5-(1-methylethyl)imidazo[1,5- a]quinoxalin-4(5 H)-one (I, U-78875) in serum is described. The validation results indicated that the present method had excellent intra- and inter-assay precision (≤ 9.5%, mean ± S.D. = 3.9±3.0%, n = 25) and accuracy (≤ 10.0%, mean ± S.D. = 3.0 ± 2.9%, n = 25), as well as improved sensitivity (2 ng/ml, using a 100-μl injection). Each chromatographic run is only 10 min and the organic solvent for the extraction of I and internal standard (U-82217) from serum was only 300 μl. The application results obtained from the SPE method were in good agreement with the advanced automated sample preparation method.
ISSN:0378-4347
DOI:10.1016/0378-4347(91)80052-E