Separation of human serum lipoproteins into three major classes by hydroxyapatite chromatography

The separation of normolipidemic male serum lipoprotein fraction, prepared by ultracentrifugal flotation, was studied on hydroxyapatite columns. Potassium phosphate buffers in the pH range 5.6–7.4 were evaluated as eluents. The three main classes of the lipoproteins (high density, low density and ve...

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Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1991-05, Vol.566 (1), p.67-76
Main Authors: Matsumoto, Ushiho, Nakayama, Hiroko, Shibusawa, Yōichi, Niimura, Takanori
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Lipoproteins, myelin
Proteins
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Summary:The separation of normolipidemic male serum lipoprotein fraction, prepared by ultracentrifugal flotation, was studied on hydroxyapatite columns. Potassium phosphate buffers in the pH range 5.6–7.4 were evaluated as eluents. The three main classes of the lipoproteins (high density, low density and very low density) can be separated on the Tiselius-type hydroxyapatite (Bio-Gel HTP DNA grade) column by elution with 75, 250 and 300 m M potassium phosphate buffer (pH 7.4), respectively.
ISSN:0378-4347
DOI:10.1016/0378-4347(91)80111-O