Determination of cytosine-β- d-arabinoside in plasma using capillary electrophoresis

An assay for the antileukaemic agent cytosine-β- d-arabinoside (ara-C) has been developed using capillary zone electrophoresis. Solid-phase extraction and on-capillary peak concentration are used to improve the detection limit. The electrophoretic separation time is less than 5 min. The limit of det...

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Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1991-07, Vol.568 (1), p.117-124
Main Authors: Lloyd, David K., Cypess, Aaron M., Wainer, Irving W.
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacology. Drug treatments
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Summary:An assay for the antileukaemic agent cytosine-β- d-arabinoside (ara-C) has been developed using capillary zone electrophoresis. Solid-phase extraction and on-capillary peak concentration are used to improve the detection limit. The electrophoretic separation time is less than 5 min. The limit of detection for ara-C in plasma is 0.5 μ M (signal-to-noise ratio = 3). The assay has been validated for the determination of ara-C in human plasma over the concentration range 1–10 μ M. The calibration curve was linear with a correlation coefficient r 2 = 0.996. At an ara-C concentration of 8 μ M the intra-day coefficient of variation was 9.1% and the inter-day coefficient of variation was 12.3%. At an ara-C concentration of 2 μ M the coefficients of variation were 15.2 and 12.0%, respectively.
ISSN:0378-4347
DOI:10.1016/0378-4347(91)80345-D