High-performance liquid chromatographic method for direct separation of 5-( p-hydroxyphenyl)-5-phenylhydantoin enantiomers using a chiral tris(4-methylbenzoate) column

After simple purification of the incubation mixture of phenytoin in isolated rat hepatocytes, 5-( p-hydroxyphenyl)-5-phenylhydantoin (p-HPPH), which formed as a major metabolite, was readily resolved to each enantiomer by direct high-performance liquid chromatography on a cellulose tris(4-methylbenz...

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Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1991-07, Vol.568 (1), p.157-163
Main Authors: Eto, Seiji, Noda, Hiroshi, Noda, Atsuko
Format: Article
Language:English
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Summary:After simple purification of the incubation mixture of phenytoin in isolated rat hepatocytes, 5-( p-hydroxyphenyl)-5-phenylhydantoin (p-HPPH), which formed as a major metabolite, was readily resolved to each enantiomer by direct high-performance liquid chromatography on a cellulose tris(4-methylbenzoate) column, with a mobile phase of ethanol—water. It was also observed that the formation of S-(−)-p-HPPH was dominant, and the S/R ratio was 11.5.
ISSN:0378-4347
DOI:10.1016/0378-4347(91)80349-H