Rapid screening method for the determination of diethylstilbestrol in edible animal tissue by column liquid chromatography with electrochemical detection

A rapid and sensitive screening method for the determination of residues of diethylstilbestrol in edible animal tissue is described. The analyte was extracted from the tissue with tert.-butyl methyl ether, reextracted with 1 M sodium hydroxide and further cleaned up by solid-phase extraction with C...

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Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1991-04, Vol.564 (2), p.477-484
Main Authors: Reuvers, Th, Perogordo, E., Jiménez, R.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Food industries
Fundamental and applied biological sciences. Psychology
Meat and meat product industries
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Summary:A rapid and sensitive screening method for the determination of residues of diethylstilbestrol in edible animal tissue is described. The analyte was extracted from the tissue with tert.-butyl methyl ether, reextracted with 1 M sodium hydroxide and further cleaned up by solid-phase extraction with C 18 cartridges. Analysis was performed by isocratic elution with a phosphate-buffered mobile phase, methanol—0.05 M phosphate buffer pH 3.5 (67:33), on a Nucleosil 5-μm C 18 column with electrochemical detection at +0.90 V. The average recovery of trans-diethylstilbestrol in spiked samples is 66%, with a standard deviation of 14% ( n = 22) in the range 0.5–2.0 μg/kg. The detection limit is 0.1–0.2 μg/kg, although at this level other compounds may interfere and give rise to false positive results.
ISSN:0378-4347
DOI:10.1016/0378-4347(91)80516-F