Equivalence of a high-performance liquid chromatographic assay and a bioassay of azithromycin in human serum samples

Two sensitive methods for the determination of the azalide antibiotic azithromycin in human serum were compared. High-performance liquid chromatography (HPLC) and a microbiological assay were simultaneously applied to 768 serum samples obtained in a clinical study. There was excellent agreement betw...

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Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1992-05, Vol.576 (2), p.358-362
Main Authors: Riedel, Klaus-Dieter, Wildfeuer, Alexander, Laufen, Heinrich, Zimmermann, Torsten
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacology. Drug treatments
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Summary:Two sensitive methods for the determination of the azalide antibiotic azithromycin in human serum were compared. High-performance liquid chromatography (HPLC) and a microbiological assay were simultaneously applied to 768 serum samples obtained in a clinical study. There was excellent agreement between the azithromycin concentrations measured by HPLC and by the bioassay. The correlation coefficient for the two methods was r 2=0.96. The precision and the sensitivity of the methods were found to be very similar.
ISSN:0378-4347
DOI:10.1016/0378-4347(92)80212-9