Simultaneous determination of fluoxetine and norfluoxetine enantiomers in biological samples by gas chromatography with electron-capture detection

An electron-capture gas chromatographic procedure was developed for the simultaneous analysis of the enantiomers of fluoxetine and norfluoxetine. The assay involves basic extraction of these enantiomers from the biological samples, followed by their conversion to diastereoisomers using the chiral de...

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Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1992-08, Vol.579 (1), p.99-106
Main Authors: Torok-Both, George A., Baker, Glen B., Coutts, Ronald T., McKenna, Kevin F., Aspeslet, Launa J.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell physiology
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
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Summary:An electron-capture gas chromatographic procedure was developed for the simultaneous analysis of the enantiomers of fluoxetine and norfluoxetine. The assay involves basic extraction of these enantiomers from the biological samples, followed by their conversion to diastereoisomers using the chiral derivatizing reagent ( S)-(−)-N-trifluoroacetylprolyl chloride. The method was utilized to detect and measure the quantity of these enantiomers in plasma and urine of patients and in liver and brain tissue of rats treated with ( R,S)-fluoxetine.
ISSN:0378-4347
DOI:10.1016/0378-4347(92)80367-Y