High-performance liquid chromatographic technique for non-derivatized leucine purification: evidence for carbon isotope fractionation

A high-performance liquid chromatographic (HPLC) method, utilizing an ion-pairing agent with a reversed-phase column, isocratic elution and ultraviolet detection, was developed for the separation of non-derivatized leucine from a mixture of amino acids. Fractionation of leucine isotopes during HPLC...

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Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1992-01, Vol.573 (1), p.11-16
Main Authors: Baumann, Patricia Quinn, Ebenstein, David B., O'Rourke, Bruce D., Nair, K.Sreekumaran
Format: Article
Language:English
Subjects:
Aminoacids, peptides. Hormones. Neuropeptides
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Proteins
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Summary:A high-performance liquid chromatographic (HPLC) method, utilizing an ion-pairing agent with a reversed-phase column, isocratic elution and ultraviolet detection, was developed for the separation of non-derivatized leucine from a mixture of amino acids. Fractionation of leucine isotopes during HPLC separation was observed, creating potential problems for collection of leucine plasma or tissue protein hydrolyzates for subsequent isotopic determinations. It was demonstrated that [ 13C]leucine and [ 12C]leucine have different retention times ( p
ISSN:0378-4347
DOI:10.1016/0378-4347(92)80467-5