Column liquid chromatographic analysis of quinine in human plasma, saliva and urine

A new simple, selective and reproducible high-performance liquid chromatographic method for the determination of quinine in plasma, saliva and urine is described. The ion-pair method was carried out on a reversed-phase C 18 column, using perchlorate ion as the counter ion and ultraviolet detection a...

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Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1993-06, Vol.616 (1), p.151-154
Main Authors: Babalola, C.P., Bolaji, O.O., Dixon, P.A.F., Ogunbona, F.A.
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacology. Drug treatments
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Summary:A new simple, selective and reproducible high-performance liquid chromatographic method for the determination of quinine in plasma, saliva and urine is described. The ion-pair method was carried out on a reversed-phase C 18 column, using perchlorate ion as the counter ion and ultraviolet detection at 254 nm. Quinine was well resolved from its major metabolite, 3-hydroxyquinine, and the internal standard, primaquine. The limit of detection was 10 ng/ml and the recovery was greater than 90% from the three biological fluids.
ISSN:0378-4347
DOI:10.1016/0378-4347(93)80482-J