Stabilization of insulin by alkylmaltosides. A. Spectroscopic evaluation

The aggregational behavior of native bovine insulin has been studied by circular dichroism and quasi elastic laser light scattering. The influence of a homologous series of alkylmaltosides on the self-association and aggregation of insulin was investigated. Significant stabilization of insulin was o...

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Bibliographic Details
Published in:International journal of pharmaceutics 1996-04, Vol.132 (1), p.107-113
Main Authors: Hovgaard, Lars, Jacobs, Harvey, Mazer, Norman A., Kim, Sung Wan
Format: Article
Language:English
Subjects:
Aggregation
Alkylmaltosides
Biological and medical sciences
Hormones. Endocrine system
Insulin
Laser light scattering
Medical sciences
Peptide
Pharmacology. Drug treatments
Self-association
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Summary:The aggregational behavior of native bovine insulin has been studied by circular dichroism and quasi elastic laser light scattering. The influence of a homologous series of alkylmaltosides on the self-association and aggregation of insulin was investigated. Significant stabilization of insulin was observed for the alkylmaltosides. Circular dichroism revealed that dodecylmaltoside was the most promising compound from a stabilizer stand point. Using dodecylmaltoside as the model compound, it was found that micelles were formed in the concentration range of the experiments and micell formation appeared to be important for the stabilization of insulin. Moreover, the results may suggest that insulin partitions into the micelles in an equimolar fashion and that insulin in this ‘mixed micellar’ form is relatively stable.
ISSN:0378-5173
1873-3476
DOI:10.1016/0378-5173(95)04371-3