Simple interface for microbore LC and electrospray ionization mass spectrometry and analysis of melphalan-alkylation sites in metallothionein

A microbore high pressure liquid chromatograph has been interfaced to a Vestec electrospray ionization source retrofitted to a Hewlett-Packard quadrupole mass spectrometer. The chief features include the absence of a splitter, the use of a second, ballast column to provide a stable flow rate across...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1995-03, Vol.13 (3), p.279-284
Main Authors: Kaltashov, Igor A., Yu, Xiaolan, Fenselau, Catherine
Format: Article
Language:English
Subjects:
Alkylating Agents
Alkylation
Analysis
Animals
Biological and medical sciences
Chromatography, Liquid
electrospray ionization mass spectrometry
General pharmacology
Hydrolysis
Mass Spectrometry
Medical sciences
Melphalan
metallothionein
Metallothionein - analysis
Microbore HPLC
Molecular Weight
Peptide Mapping
Peptides - analysis
Pharmacology. Drug treatments
Rabbits
Spectrophotometry, Ultraviolet
Trypsin
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Description
Summary:A microbore high pressure liquid chromatograph has been interfaced to a Vestec electrospray ionization source retrofitted to a Hewlett-Packard quadrupole mass spectrometer. The chief features include the absence of a splitter, the use of a second, ballast column to provide a stable flow rate across the gradient, and an in-line UV detector. The system was evaluated for analysis of peptide mixtures and applied to identification of drug-modified peptides released by tryptic digestion of drug-alkylated metallothionein.
ISSN:0731-7085
1873-264X
DOI:10.1016/0731-7085(95)01271-L