Electron microscopic studies of negatively stained liposomes and membrane intrinsic polypeptide

High resolution electron microscopy has been applied to the study of the permeability properties of liposomes reconstituted with lens fibre protein. The experiments showed that liposomes formed in the presence of main intrinsic polypeptide (MIP) of bovine lens fibres appeared to become modified by c...

Full description

Saved in:
Bibliographic Details
Published in:Micron and microscopica acta 1990, Vol.21 (1-2), p.91-99
Main Authors: Tickner, A.L., Lea, E.J.A., Horne, R.W.
Format: Article
Language:English
Subjects:
electron microscopy
junctional protein
Lens
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:High resolution electron microscopy has been applied to the study of the permeability properties of liposomes reconstituted with lens fibre protein. The experiments showed that liposomes formed in the presence of main intrinsic polypeptide (MIP) of bovine lens fibres appeared to become modified by changes in shape; also the bilayers seen in the electron micrographs of negatively stained specimens showed evidence of some structural changes or granularity. The morphological appearance of liposomes formed in the presence of MIP were interpreted as being consistent with the results of experiments performed by other methods.
ISSN:0739-6260
DOI:10.1016/0739-6260(90)90009-5