Xanthine oxidase activity in bronchoalveolar lavage fluid from patients with chronic obstructive pulmonary disease

Chronic obstructive pulmonary disease (COPD) is a serious respiratory pathology characterized by irreversible limitation of expiratory flow and includes chronic obstructive bronchitis, chronic airflow limitation, and emphysema. To determine whether xanthine oxidase activity increased in the airspace...

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Bibliographic Details
Published in:Free radical biology & medicine 1996, Vol.21 (2), p.147-155
Main Authors: Pinamonti, Silvano, Muzzoli, Mariavittoria, Chicca, Milvia C., Papi, Alberto, Ravenna, Franco, Fabbri, Leonardo M., Ciaccia, Adalberto
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Bronchoalveolar lavage fluid
Bronchoalveolar Lavage Fluid - chemistry
Chronic obstructive pulmonary disease
Cytochrome c
Cytochrome c Group - metabolism
DNA - analysis
DNA Damage
Female
Fluorometric analysis of DNA unwinding
Humans
Kinetics
Lung Diseases, Obstructive - enzymology
Male
Middle Aged
Mutagens - analysis
Oxidation-Reduction
Oxygen radicals
Spectrometry, Fluorescence
Superoxides - metabolism
Uric Acid - metabolism
Uricase
Xanthine oxidase
Xanthine Oxidase - metabolism
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Summary:Chronic obstructive pulmonary disease (COPD) is a serious respiratory pathology characterized by irreversible limitation of expiratory flow and includes chronic obstructive bronchitis, chronic airflow limitation, and emphysema. To determine whether xanthine oxidase activity increased in the airspaces of COPD patients, we examined bronchoalveolar lavage fluid (BAL) from COPD patients recruited during a 2-year clinical study. Filtered BAL supernatant from COPD patients and healthy nonsmoking controls was examined by fluorometric analysis of DNA unwinding (FADU) and spectrophotometric assays (cytochrome c reduction kinetics and uric acid kinetics). Compared to controls, filtered BAL supernatant of subjects with COPD exhibited a detectable clastogenic activity probably related to superoxide production. The method of BAL preparation as an acellular system strongly suggests that superoxide production may be due to xanthine oxidase activity.
ISSN:0891-5849
1873-4596
DOI:10.1016/0891-5849(96)00030-5