Molecular recognition and assembly sequences involved in the subfamily-specific assembly of voltage-gated K + channel subunit proteins

We are analyzing features of the K + channel subunit proteins that are critical for function and regulation of these proteins. Our studies show biochemically that subunit proteins from the Shaker and Shaw subfamilies fail to assemble into a heteromultimer. The basis for this incompatibility is the s...

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Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Mass.), 1995-03, Vol.14 (3), p.625-633
Main Authors: Shen, N.Vivienne, Pfaffinger, Paul J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Aplysia
Cloning, Molecular
Conserved Sequence
Ion Channel Gating
Macromolecular Substances
Molecular Sequence Data
Mutagenesis, Site-Directed
Neurons - physiology
Polymerase Chain Reaction
Potassium Channels - biosynthesis
Potassium Channels - physiology
Protein Biosynthesis
Recombinant Proteins - biosynthesis
Sequence Homology, Amino Acid
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Summary:We are analyzing features of the K + channel subunit proteins that are critical for function and regulation of these proteins. Our studies show biochemically that subunit proteins from the Shaker and Shaw subfamilies fail to assemble into a heteromultimer. The basis for this incompatibility is the sequences contained within the T1 assembly domain. For a subunit protein to heteromultimerize with a Shaker subunit protein, two regions within the T1 domain, A and B, must be of the Shaker subtype. Finally, we show that the incompatibility of a Shaw A region for assembly with a Shaker protein depends upon the composition of a 30 amino acid conserved sequence in the A region.
ISSN:0896-6273
1097-4199
DOI:10.1016/0896-6273(95)90319-4