Selective enzymatic reactions using microemulsion-based gels

Aliphatic acids were allowed to react at 298.2 K with racemic 2-octanol catalysed by the commercial lipase of Chromobacterium viscosum immobilized in microemulsion-based gelatin gels. The lipase was solubilized in microemulsions having different microstructures: a water-in-oil microemulsion based on...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 1995-03, Vol.4 (2), p.121-127
Main Authors: Backlund, Sune, Eriksson, Folke, Kanerva, Liisa T., Rantala, Maria
Format: Article
Language:English
Subjects:
Enzyme catalysis
Esterification
Microemulsion-based gels
Soybean lecithin
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Summary:Aliphatic acids were allowed to react at 298.2 K with racemic 2-octanol catalysed by the commercial lipase of Chromobacterium viscosum immobilized in microemulsion-based gelatin gels. The lipase was solubilized in microemulsions having different microstructures: a water-in-oil microemulsion based on waterhexanesodium 1,4-bis(2-ethylhexyl)sulfosuccinate and a microemulsion having bicontinuous structure based on water/ethanol-hexadecane-soybean lecithin. The syntheses were carried out in various hydrophobic solvents using different initial reactant concentrations. The immobilized lipase exhibits stereoselective action which, however, decreases with use. Enantiomeric excess values in this work are the same or somewhat higher compared with syntheses performed in organic solvents.
ISSN:0927-7765
1873-4367
DOI:10.1016/0927-7765(94)01162-X