Electrochemical immunosensors for determination of the pesticides 2,4-dichlorophenoxyacetic and 2,4,5-tricholorophenoxyacetic acids

Two immunochemical sensors are described for detection of the pesticides 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). The assay monitors the competitive binding of free pesticide and pesticide-peroxidase conjugate with antibodies immobilized on a graphite e...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1996, Vol.11 (1), p.179-185
Main Authors: Dzantiev, B.B, Zherdev, A.V, Yulaev, M.F, Sitdikov, R.A, Dmitrieva, N.M, Moreva, I.Yu
Format: Article
Language:English
Subjects:
4-dichlorophenoxyacetic acid
5-T
5-trichlorophenoxyacetic acid
D
immunoenzyme electrode
pesticides
potentiometric sensor
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Summary:Two immunochemical sensors are described for detection of the pesticides 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). The assay monitors the competitive binding of free pesticide and pesticide-peroxidase conjugate with antibodies immobilized on a graphite electrode, by measurement of peroxidase activity in the immune complexes on the electrode surface. 5-aminosalicyclic acid and H 2O 2 are used as substrates. An automated potentiometric device measures changes in redox potential during the peroxidase reaction. The assay parameters were optimized to achieve detection limits of 40 ng/ml 2,4-D and 50 ng/ml 2,4,5-T (in both water and serum). The total time required to perform the assay (including electrode regeneration) was 12 min, compared to about 2 h for solid-phase enzyme immunoassays. The electrode life extends to 60 sequential measurements. The sensors therefore appear suitable for medical and ecological applications.
ISSN:0956-5663
1873-4235
DOI:10.1016/0956-5663(96)83725-0