Monitoring of cellular behaviour by impedance measurements on interdigitated electrode structures

A new method for on-line and real-time monitoring of concentration, growth and physiological state of cells in culture is described. This biosensor is based on impedance measurements of adherently growing cells on interdigitated electrode structures (IDES). The measurements can be performed for seve...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1997, Vol.12 (1), p.29-41
Main Authors: Ehret, R., Baumann, W., Brischwein, M., Schwinde, A., Stegbauer, K., Wolf, B.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
Cell Survival
cellular biosensor
Cercopithecus aethiops
cytotoxicity testing
Electric Impedance
Electrodes
Fundamental and applied biological sciences. Psychology
Humans
impedance
interdigitated electrode structures
Male
Methods. Procedures. Technologies
Octoxynol - pharmacology
PhysicoControl-Microsystem
Sensitivity and Specificity
serum components
Various methods and equipments
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Summary:A new method for on-line and real-time monitoring of concentration, growth and physiological state of cells in culture is described. This biosensor is based on impedance measurements of adherently growing cells on interdigitated electrode structures (IDES). The measurements can be performed for several days as there is no detectable electrical influence on the cells. The versatility of this new sensor is shown with some exemplary experiments. Cell density, growth and long-term behaviour of cells on the electrodes clearly change the impedance of the IDES. Both, the global influence of serum components (deprivation of foetal bovine serum) and the toxic effects of heavy metal ions (cadmium) result in changes of the sensor signal and can be visualized.
ISSN:0956-5663
1873-4235
DOI:10.1016/0956-5663(96)89087-7