Transketolase from Escherichia coli: A practical procedure for using the biocatalyst for asymmetric carbon-carbon bond synthesis

A practical procedure is reported for the use of the enzyme transketolase, from Escherichia coli, for asymmetric carbon-carbon bond synthesis. The reactions with the biocatalyst are conveniently carried out, on a gram scale, in unbuffered aqueous media by employing a pH autotitrator. An improved lar...

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Bibliographic Details
Published in:Tetrahedron: asymmetry 1996-08, Vol.7 (8), p.2185-2188
Main Authors: Morris, K.Gall, Smith, Mark E.B., Turner, Nicholas J., Lilly, Malcolm D., Mitra, Robin K., Woodley, John M.
Format: Article
Language:English
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Summary:A practical procedure is reported for the use of the enzyme transketolase, from Escherichia coli, for asymmetric carbon-carbon bond synthesis. The reactions with the biocatalyst are conveniently carried out, on a gram scale, in unbuffered aqueous media by employing a pH autotitrator. An improved large scale synthesis of hydroxypyruvate is also reported. A practical protocol is reported for the use of E. coli transketolase in asymmetric synthesis.
ISSN:0957-4166
1362-511X
DOI:10.1016/0957-4166(96)00266-2