Production of cellulolytic enzymes by Neurospora sitophila grown on cellulosic materials

Cellulases and β-glucosidase were produced by Neurospora sitophila cultured in media containing corn stover or sugar cane bagasse as carbon sources. The effects of initial medium pH, organic nitrogen source and Tween 80 on enzyme activity were investigated. Values of pH above 6·5 were found to favou...

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Bibliographic Details
Published in:Bioresource technology 1992, Vol.39 (3), p.277-283
Main Authors: Oguntimein, Gbekeloluwa, Vlach, Dagmar, Moo-Young, Murray
Format: Article
Language:English
Subjects:
bagasse
Biological and medical sciences
Biotechnology
Cellulases
corn stover
Enzyme engineering
Fermentation
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Neurospora sitophila
Production of selected enzymes
wheat straw
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Summary:Cellulases and β-glucosidase were produced by Neurospora sitophila cultured in media containing corn stover or sugar cane bagasse as carbon sources. The effects of initial medium pH, organic nitrogen source and Tween 80 on enzyme activity were investigated. Values of pH above 6·5 were found to favour enzyme production. Defatted sesame seed flour increased both carboxymethyl-cellulase and filter-paper activities, while yeast extract enhanced β-glucosidase activity. Tween 80 at a concentration of 0·1% (v/v) increased all the enzyme activities.
ISSN:0960-8524
1873-2976
DOI:10.1016/0960-8524(92)90217-L