Enzymatic synthesis of dodecyl.β- d-glucopyranoside catalyzed by Candida molischiana 35M5N β-glucosidase

The enzymatic synthesis of dodecyl. β- d-glucopyrano-side was studied by using the β-glucosidase from Candida molischiana 35M5N in the examination of transglucosylation and hydrolysis reversion reactions. On account of cost considerations, the condensation reaction was optimized. The effects of the...

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Bibliographic Details
Published in:Bioresource technology 1995, Vol.53 (3), p.263-267
Main Authors: Gueguen, Yannick, Chemardin, Patrick, Pommares, Patrick, Arnaud, Alain, Galzy, Pierre
Format: Article
Language:English
Subjects:
alkyl glucosides
beta-glucosidase
Biological and medical sciences
biosynthesis
Biotechnology
Candida
chemical reactions
condensation reaction
Dodecyl. β- d-glucopyranoside
Enzyme engineering
Fundamental and applied biological sciences. Psychology
glucosides
hydrolysis
Methods. Procedures. Technologies
Miscellaneous
surfactants
transglucosylation
β-glucosidase
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Summary:The enzymatic synthesis of dodecyl. β- d-glucopyrano-side was studied by using the β-glucosidase from Candida molischiana 35M5N in the examination of transglucosylation and hydrolysis reversion reactions. On account of cost considerations, the condensation reaction was optimized. The effects of the extraction solvent, the initial 1-dodecanol and glucose concentrations, the temperature and the incubation time were studied. The optimal conditions for the enzymatic synthesis of dodecyl. β- d-glucopyranoside by the condensation reaction were: 90% (v/v) 1-dodecanol initial concentration; glucose concentration (50 m m), temperature 40°C and reaction time 20 h.
ISSN:0960-8524
1873-2976
DOI:10.1016/0960-8524(95)00095-V