Direct characterization of solid phase resin-bound molecules by mass spectrometry

In this work we demonstrate that analytes, covalently linked to a polymeric support through a photolabile linker, can be directly analyzed by matrix-assisted laser desorption/ionization (MALDI). Mass spectral analysis is performed in a single step requiring no pretreatment of the sample to induce cl...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters 1996-04, Vol.6 (8), p.979-982
Main Authors: Fitzgerald, Michael C., Harris, Ken, Shevlin, Charles G., Siuzdak, Gary
Format: Article
Language:English
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Summary:In this work we demonstrate that analytes, covalently linked to a polymeric support through a photolabile linker, can be directly analyzed by matrix-assisted laser desorption/ionization (MALDI). Mass spectral analysis is performed in a single step requiring no pretreatment of the sample to induce cleavage from the support. Our results show that the UV laser light in the MALDI experiment can be used to simultaneously promote an analyte's photolytic cleavage from a solid support and its gas phase ionization for subsequent mass spectral analysis. In this manner, MALDI facilitates the dissociation and identification of resin-bound analytes in a single analytical procedure without the need for any prior chemical treatment. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry has been used to facilitate the dissociation and identification of resin-bound analytes in a single analytical procedure.
ISSN:0960-894X
1464-3405
DOI:10.1016/0960-894X(96)00152-7