Co-electropolymerization of a viologen oligomer and lipoamide dehydrogenase on an electrode surface. Application to cofactor regeneration

We report here a novel enzyme electrode obtained by electrochemically copolymerization of a redox enzyme with an oligomeric orgaric redox mediator on the surface of a glassy carbon electrode. This electrode serves as an efficient catalyst for the regeneration of the cofactor in enzymatic syntheses r...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters 1996-07, Vol.6 (14), p.1663-1666
Main Authors: Leonida, Mihaela Draganoiu, Fry, Albert J., Sobolov, Susan B., Voivodov, Kamen I.
Format: Article
Language:English
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Summary:We report here a novel enzyme electrode obtained by electrochemically copolymerization of a redox enzyme with an oligomeric orgaric redox mediator on the surface of a glassy carbon electrode. This electrode serves as an efficient catalyst for the regeneration of the cofactor in enzymatic syntheses requiring NADH. The reaction shows a relatively short induction period and the rate of product formation is higher than for similar electrodes upon which the enzyme and redox mediator are physically trapped under a Nafion membrane. Graphic
ISSN:0960-894X
1464-3405
DOI:10.1016/0960-894X(96)00298-3