Chemical indicators as enhancers of the chemiluminescent luminol-H2O2-horseradish peroxidase reaction

The chemiluminescent emission from the luminol-H2O2-horseradish peroxidase system was enhanced between 2 and 80 times, during the first 5 min, by chemical indicators, such as phenolphthalein, cresolphthalein, phenol red, cresol red, benzidine and o-tolidine. Phenol derivatives showed emission maxima...

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Bibliographic Details
Published in:Journal of photochemistry and photobiology. A, Chemistry. Chemistry., 1995-03, Vol.87 (2), p.99-103
Main Authors: NAVAS DIAZ, A, GARCIA SANCHEZ, F, GONZALEZ GARCIA, J. A
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemical indicators
Chemiluminescence
Fundamental and applied biological sciences. Psychology
Luminol-H2O2-horseradish peroxidase
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Summary:The chemiluminescent emission from the luminol-H2O2-horseradish peroxidase system was enhanced between 2 and 80 times, during the first 5 min, by chemical indicators, such as phenolphthalein, cresolphthalein, phenol red, cresol red, benzidine and o-tolidine. Phenol derivatives showed emission maxima at pH 8.5, while aniline derivatives showed emission maxima at pH 10.5. The enhancers with a methyl group at the ortho or para position to the OH and NH2 groups in the benzene ring showed an inhibitory effect when their concentrations were increased.
ISSN:1010-6030
1873-2666
DOI:10.1016/1010-6030(94)03970-6