Electro-optical investigation of thylakoids and PS II fragments treated with trypsin

The surface electric properties of thylakoids and Photosystem II-enriched particles subjected to proteolytic treatment were investigated by electric light scattering and microelectrophoresis. The observed variation of the charge asymmetry (permanent dipole moment) and electric polarizability upon tr...

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Bibliographic Details
Published in:Journal of photochemistry and photobiology. B, Biology Biology, 1994, Vol.23 (1), p.19-25
Main Authors: Taneva, Stefka G., Busheva, Mira C., Dobrikova, Anelia G., Petkanchin, Ivana B.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell structures and functions
Chloroplast, photosynthetic membrane and photosynthesis
Electric polarizability
Electrophoretic mobility
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Permanent dipole moment
PS II particles
Thylakoids
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Summary:The surface electric properties of thylakoids and Photosystem II-enriched particles subjected to proteolytic treatment were investigated by electric light scattering and microelectrophoresis. The observed variation of the charge asymmetry (permanent dipole moment) and electric polarizability upon treatment of thylakoids with trypsin in a wide concentration range and different time of enzyme action, associated with the removal of positively charged polypeptides from the thylakoid membranes, correlated with the increase of the electrokinetic charge. The higher transversal charge asymmetry of trypsin-treated Photosystem II fragments could be connected with destacking of the paired membranes upon treatment with trypsin and formation of unpaired single membrane fragments.
ISSN:1011-1344
1873-2682
DOI:10.1016/1011-1344(93)06976-A