Identification of a new fragment ion type in the collision-induced dissociation spectra of peptides: formation of a 2-16 ions

The identification of an ion observed in the high-energy collision-induced dissociation spectra of several model peptides is reported. The ion, observed at m/z 99 for the peptide pentaalanine (Ala 5) and designated a 2-16, is shown to have an elemental formula of C 5H 9NO by high-resolution peak mat...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 1993-05, Vol.4 (5), p.424-427
Main Authors: Thornburg, K.R., Schey, K.L., Knapp, D.R.
Format: Article
Language:English
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Summary:The identification of an ion observed in the high-energy collision-induced dissociation spectra of several model peptides is reported. The ion, observed at m/z 99 for the peptide pentaalanine (Ala 5) and designated a 2-16, is shown to have an elemental formula of C 5H 9NO by high-resolution peak matching. The precursor ion spectrum of the a 2-16 ion and product ion spectra of the a 2 and the a 2 + 1 ions for Ala 5 suggest that this ion is formed by the loss of 17 u (presumably NH 3) from the a 2 + 1 ion and, to a lesser extent, by loss of 28 u (presumably CO) from the b 2-16 ion. On the basis of the data presented and other experimental evidence, a structure and mechanism for the formation of the a 2-16 ion is proposed.
ISSN:1044-0305
1879-1123
DOI:10.1016/1044-0305(93)85008-L