Fluorescence spectroscopy in biological and medical research

Fluorescence spectroscopy is one of the most sensitive and versatile tools in medical, biological and biochemical research. Here we discuss the use of (1) polarized fluorescence spectroscopy to study the conformational dynamics of proteins, (2) fluorescence recovery after photobleaching to study lat...

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Bibliographic Details
Published in:International journal of radiation applications and instrumentation. Part C, Radiation physics and chemistry Radiation physics and chemistry, 1988, Vol.32 (3), p.457-464
Main Authors: Yguerabide, Juan, Yguerabide, E.E.
Format: Article
Language:English
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Summary:Fluorescence spectroscopy is one of the most sensitive and versatile tools in medical, biological and biochemical research. Here we discuss the use of (1) polarized fluorescence spectroscopy to study the conformational dynamics of proteins, (2) fluorescence recovery after photobleaching to study lateral mobility of proteins and lipids in biological cell membranes and (3) excitation energy transfer to measure distances between interesting sites in macromolecules and biological membranes.
ISSN:1359-0197
1878-1020
DOI:10.1016/1359-0197(88)90049-5