Indocyanine green effect on cultured human retinal pigment epithelial cells: implication for macular hole surgery

PURPOSE: To evaluate potential toxic effects of indocyanine green dye on cultured human retinal pigment epithelial cells. METHODS: Controlled laboratory experiment. Cultured human retinal pigment epithelial cells were exposed to balanced saline solution, balanced saline solution with endoilluminatio...

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Published in:American journal of ophthalmology 2001-09, Vol.132 (3), p.433-435
Main Authors: Sippy, Brian D, Engelbrecht, Nicholas E, Hubbard, George Baker, Moriarty, Siobhan E, Jiang, Shunai, Aaberg, Thomas M, Grossniklaus, Hans E, Sternberg, Paul
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cells, Cultured
Coloring Agents - pharmacology
Enzyme-Linked Immunosorbent Assay
Humans
Indocyanine Green - pharmacology
Medical sciences
Mitochondria - drug effects
Mitochondria - enzymology
Oxidoreductases - metabolism
Pigment Epithelium of Eye - drug effects
Pigment Epithelium of Eye - enzymology
Pigment Epithelium of Eye - ultrastructure
Retinal Perforations - surgery
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgery of the eye and orbit
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Summary:PURPOSE: To evaluate potential toxic effects of indocyanine green dye on cultured human retinal pigment epithelial cells. METHODS: Controlled laboratory experiment. Cultured human retinal pigment epithelial cells were exposed to balanced saline solution, balanced saline solution with endoillumination, indocyanine green or indocyanine green with endoillumination. Cells were evaluated by light microscopy, electron microscopy, and a mitochondrial dehydrogenase assay. RESULTS: Retinal pigment epithelial cells exposed to indocyanine green showed no histologic or ultrastructural changes. Those exposed to indocyanine green alone or indocyanine green plus light demonstrated a significant decrease in mitochondrial enzyme activity ( P = 0.0002 and 0.005, respectively). CONCLUSION: Brief exposure of cultured human retinal pigment epithelial cells to indocyanine green results in decreased mitochondrial enzyme activity but does not appear to influence cellular morphology or ultrastructure.
ISSN:0002-9394
1879-1891
DOI:10.1016/S0002-9394(01)01061-3