Digoxinlike Immunoreactive Factor Isolated From Human Pleural Fluids is Structurally Similar to Digoxin

To further define the chemical structure of human endogenous digoxinlike immunoreactive factors (DLIF) we used human pleural effusions as a source of the substance. Digoxinlike immunoreactive factor activity was detected by radioimmunoassay in the pleural fluid of each of four patients; average conc...

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Bibliographic Details
Published in:The American journal of the medical sciences 1997-07, Vol.314 (1), p.28-30
Main Authors: Weinberg, U.z.i., Shllo, Lotan, Ooley, Sara, Shapiro, Menachem S., Rabinowitz, Ronen, Shenkman, Louis
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cardiotonic agents
Cardiovascular system
Digoxin
Digoxinlike immunoreactive factor
Medical sciences
Ouabain
Pharmacology. Drug treatments
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Summary:To further define the chemical structure of human endogenous digoxinlike immunoreactive factors (DLIF) we used human pleural effusions as a source of the substance. Digoxinlike immunoreactive factor activity was detected by radioimmunoassay in the pleural fluid of each of four patients; average concentration was 0.35 ng/mL. The chemical profile of DLIF was determined by initial extraction and concentration of DLIF by ion exchange chromatography followed by reverse phase-high-pressure liquid chromatography (RP-HPLC) separation and purification. Using high-pressure liquid chromatography cochromatography of DLIF, together with several radioactively marked glycosides, we observed a single peak of DLIF activity that was chro- matographically identical to digoxin. The present study further supports the recent finding that DLIF is related structurally to the cardiac glycosides, and for the first time it has been proven that DLIF is present in pleural fluids
ISSN:0002-9629
1538-2990
DOI:10.1016/S0002-9629(15)40152-1