Viscometric assay for determining lysozyme activity

A method was developed for determining the activity of lysozyme using two pipettes as a viscometer. The proposed viscometric assay uses carboxymethylchitin as a substrate and measures the enzyme activity at 22°C. The method requires only simple manipulations with the use of readily available laborat...

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Bibliographic Details
Published in:Analytica chimica acta 1989, Vol.219 (1), p.123-126
Main Authors: Yamaoka, Keiko, Ishii, Toshihiro, Natsume, Yoshiaki, Moriyama, Hiroyoshi
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Hydrolases
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Summary:A method was developed for determining the activity of lysozyme using two pipettes as a viscometer. The proposed viscometric assay uses carboxymethylchitin as a substrate and measures the enzyme activity at 22°C. The method requires only simple manipulations with the use of readily available laboratory equipment and the determination of enzyme activity is rapid.
ISSN:0003-2670
1873-4324
DOI:10.1016/S0003-2670(00)80339-6