Chemiluminescence detection of haem-containing microsomal proteins

In the purification and characterization of cytochrome P-450 isoenzymes from rat liver microsomes, a luminol-mediated chemiluminescence in microsomes after addition of peroxides was discovered. The pH dependence of the kinetics of the chemiluminescence reaction was investigated and the reactions con...

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Bibliographic Details
Published in:Analytica chimica acta 1989, Vol.227 (1), p.49-55
Main Authors: Jansen, E.H.J.M., Reinerink, E.J.M., Van Den Berg, R.H.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Miscellaneous
Proteins
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Summary:In the purification and characterization of cytochrome P-450 isoenzymes from rat liver microsomes, a luminol-mediated chemiluminescence in microsomes after addition of peroxides was discovered. The pH dependence of the kinetics of the chemiluminescence reaction was investigated and the reactions conditions were optimized with respect to the peroxide and luminol concentrations. The detection limits appear to be a factor of 10 3 lower than those obtained with other techniques. The speed of detection was also improved substantially. The nature of the strong chemiluminescence was further investigated by (partial) purification of the cytochrome P-450 isoenzymes. It appeared that the chemiluminescence activity was closed related to the presence of cytochrome P-450.
ISSN:0003-2670
1873-4324
DOI:10.1016/S0003-2670(00)82644-6