Determination of the antibacterial norfloxacin in human urine and serum samples by solid-phase spectrofluorimetry

A method for the determination of trace amounts of norfloxacin has been developed, based on solid-phase spectrofluorimetry. The relative fluorescence intensity (RFI) of norfloxacin fixed on Sephadex SP C-25 gel was measured directly after packing the gel beads in a 1 mm silica cell, using a solid-ph...

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Bibliographic Details
Published in:Analytica chimica acta 2001-10, Vol.444 (2), p.279-286
Main Authors: Vı́lchez, J.L, Ballesteros, O, Taoufiki, J, Sánchez-Palencia, G, Navalón, A
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
General pharmacology
Medical sciences
Norfloxacin
Pharmacology. Drug treatments
Serum analysis
Solid-phase spectrofluorimetry
Urine analysis
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Summary:A method for the determination of trace amounts of norfloxacin has been developed, based on solid-phase spectrofluorimetry. The relative fluorescence intensity (RFI) of norfloxacin fixed on Sephadex SP C-25 gel was measured directly after packing the gel beads in a 1 mm silica cell, using a solid-phase attachment. The wavelengths of excitation and emission were 272 and 446 nm, respectively. Using a sample volume of 1000 ml, the linear concentration range of application was 0.1–4.0 ng ml −1 of norfloxacin, with a relative standard deviation (R.S.D.) of 1.4% (for a level of 2.0 ng ml −1) and a detection limit of 0.04 ng ml −1. The method was applied to the determination of norfloxacin in human urine and serum samples. It was validated applying the standard addition methodology and using HPLC as a reference method. Recovery levels of the method reached 100% in all cases.
ISSN:0003-2670
1873-4324
DOI:10.1016/S0003-2670(01)01238-7