Cholera toxin-induced modulation of gene expression: elucidation via cDNA microarray for rational cell-based sensor design

Cell-based biosensors utilize functional changes in cellular response to identify the biological threats in a physiological relevant manner. Cell-based sensors have been used for a wide array of applications including toxicological assessment and drug-screening. In this paper, we utilize DNA arrays...

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Bibliographic Details
Published in:Analytica chimica acta 2002-04, Vol.457 (1), p.97-108
Main Authors: Lin, Hsingchi J., Charles, P.T., Andreadis, Joanne D., Churilla, A.M., Stenger, David A., Pancrazio, J.J.
Format: Article
Language:English
Subjects:
cDNA microarray
Cholera
Enterotoxin
Epithelial
False positive
Gene expression
Lymphoma
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Summary:Cell-based biosensors utilize functional changes in cellular response to identify the biological threats in a physiological relevant manner. Cell-based sensors have been used for a wide array of applications including toxicological assessment and drug-screening. In this paper, we utilize DNA arrays to identify differential gene expression events induced by toxin exposure for the purpose of developing a reporter gene assay system compatible with insertion into a cell-based sensor platform. HT29, an intestine epithelial cell line, was used as a cell model to study the cholera toxin (CT)-induced host cell modulation using DNA array analysis. A false positive model was generated from analysis of housekeeping genes in untreated control experiments to characterize our system and to minimize the number of false positives in the data. Threshold probability scores (−3.72), which gives
ISSN:0003-2670
1873-4324
DOI:10.1016/S0003-2670(01)01353-8