Determination of benzo[a]pyrene in cigarette smoke condensate by liquid chromatography on amberlite xad-2

A method is described for the determination of benzo[a]pyrene in cigarette smoke condensate which utilizes chromatographic fractionation on Amberlite XAD-2. PAH are initially separated by step-wise gradient elution, progressing from reverse to normal-phase modes of operation. Other separation steps...

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Bibliographic Details
Published in:Analytica chimica acta 1980-01, Vol.115, p.229-238
Main Authors: Robinson, Jack L., Marshall, Monte A., Draganjac, Mark E., Noggle, Lawrence C.
Format: Article
Language:English
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Summary:A method is described for the determination of benzo[a]pyrene in cigarette smoke condensate which utilizes chromatographic fractionation on Amberlite XAD-2. PAH are initially separated by step-wise gradient elution, progressing from reverse to normal-phase modes of operation. Other separation steps involve automated column chromatography on silica gel and thin-layer chromatography on 20% acetylated cellulose. Benzo[a]pyrene is finally determined by u.v. spectrophotometry and liquid scintillation counting of 14C—benzo[a]pyrene tracer. Results obtained compare favorably with those of the more traditional liquid—liquid extraction methods.
ISSN:0003-2670
1873-4324
DOI:10.1016/S0003-2670(01)93161-7