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Determination of lactate dehydrogenase in serum by using a pyruvate sensor

Pyruvate produced by lactate dehydrogenase (LDH)-catalyzed oxidation of lactate is determined by the sensor based on pyruvate oxidase described previously. Thus LDH activity (0.025–0.18 I.U. ml -1) can be determined. The time required for the assay is 6 min.

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Bibliographic Details
Published in:Analytica chimica acta 1982-03, Vol.135 (2), p.355-357
Main Authors: Minoura, Norihiko, Yamada, Sumio, Karube, Isao, Kubo, Izumi, Suzuki, Shuichi
Format: Article
Language:English
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Description
Summary:Pyruvate produced by lactate dehydrogenase (LDH)-catalyzed oxidation of lactate is determined by the sensor based on pyruvate oxidase described previously. Thus LDH activity (0.025–0.18 I.U. ml -1) can be determined. The time required for the assay is 6 min.
ISSN:0003-2670
1873-4324
DOI:10.1016/S0003-2670(01)93919-4