Polyelectrolyte-stabilized biosensors based on macroporous carbon electrode

In this work, polyelectrolyte-stabilized enzymes are used for the construction of stable biosensors utilizing a simple manufacture procedure. The stabilized enzyme is immobilized by physical adsorption into activated macroporous and conductive carbon material, which serves as the immobilization matr...

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Bibliographic Details
Published in:Analytica chimica acta 2002-09, Vol.467 (1), p.217-223
Main Authors: Dimakis, Vaggelis T, Gavalas, Vasilis G, Chaniotakis, Nikolas A
Format: Article
Language:English
Subjects:
Biosensor
Polyelectrolyte
Porous carbon
Stabilization
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Summary:In this work, polyelectrolyte-stabilized enzymes are used for the construction of stable biosensors utilizing a simple manufacture procedure. The stabilized enzyme is immobilized by physical adsorption into activated macroporous and conductive carbon material, which serves as the immobilization matrix and the electrochemical transducer. The amount of polyelectrolyte used is optimized with respect to sensor’s sensitivity and stability. The optimized biosensors exhibit high operational stability and good sensor-to-sensor reproducibility.
ISSN:0003-2670
1873-4324
DOI:10.1016/S0003-2670(02)00377-X