Disposable test plates with tyrosinase and β-glucosidases for cyanide and cyanogenic glycosides

Colorimetric methods for the determination of cyanide and cyanogenic glycosides (amygdalin and linamarin) are developed. In the presence of dissolved oxygen, tyrosinase catalyzes the oxidation of l-tyrosine to dark melanin via red dopachrome. This coloring reaction is inhibited by cyanide, which is...

Full description

Saved in:
Bibliographic Details
Published in:Analytica chimica acta 2000-03, Vol.408 (1), p.233-240
Main Authors: Tatsuma, Tetsu, Komori, Kikuo, Yeoh, Hock-Hin, Oyama, Noboru
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Others
Various methods and equipments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Colorimetric methods for the determination of cyanide and cyanogenic glycosides (amygdalin and linamarin) are developed. In the presence of dissolved oxygen, tyrosinase catalyzes the oxidation of l-tyrosine to dark melanin via red dopachrome. This coloring reaction is inhibited by cyanide, which is added or liberated from a cyanogenic glycoside as a result of hydrolysis catalyzed by a β-glucosidase. This inhibition is utilized to determine cyanide and the cyanogenic glycosides. A spectrophotometric method is quantitative, and its typical dynamic range is 3 μM to 0.1 mM. Test plates that are coated by the films containing tyrosinase, a β-glucosidase, l-tyrosine, and poly(ethyleneoxide) are also prepared as semi-quantitative devices, and their typical dynamic range is 10 μM to 10 mM. This is expected to be an inexpensive, safe, and convenient method for end-users.
ISSN:0003-2670
1873-4324
DOI:10.1016/S0003-2670(99)00744-8