Expression and localization of ferritin mRNA in ameloblasts of rat incisor

At the maturation stage, the ameloblasts of the rat incisor incorporate iron, supplied through the bloodstream, and deposit it into the surface layer of the enamel. In this unique iron transport system, ferritin functions as a transient iron reservoir in the cells. Here the expression of ferritin mR...

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Bibliographic Details
Published in:Archives of oral biology 1998-05, Vol.43 (5), p.367-378
Main Authors: Miyazaki, Yumiko, Sakai, Hideaki, Shibata, Yasuaki, Shibata, Mitsue, Mataki, Shiro, Kato, Yuzo
Format: Article
Language:English
Subjects:
ameloblasts
Ameloblasts - chemistry
Ameloblasts - metabolism
Animals
Dental Enamel Proteins - analysis
Dental Enamel Proteins - biosynthesis
Dental Enamel Proteins - chemistry
Electrophoresis, Polyacrylamide Gel
Enamel Organ - metabolism
ferritin
Ferritins - analysis
Ferritins - biosynthesis
Ferritins - chemistry
Immunoblotting
Immunohistochemistry
In Situ Hybridization
Incisor
Male
Polymerase Chain Reaction
Rats
Rats, Wistar
RNA Probes
RNA, Messenger - analysis
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Summary:At the maturation stage, the ameloblasts of the rat incisor incorporate iron, supplied through the bloodstream, and deposit it into the surface layer of the enamel. In this unique iron transport system, ferritin functions as a transient iron reservoir in the cells. Here the expression of ferritin mRNA and its localization in the rat enamel organ was examined. Among various tissues, the enamel organ showed the highest expression for both ferritin H- and L-chain mRNA, as quantified by reverse transcription–polymerase chain reaction. In situ hybridization using digoxigenin-labelled cRNA probes for each chain demonstrated that both ferritin H- and L-chain mRNA were abundantly expressed in presecretory and secretory ameloblasts. The intensity of the positive hybridization signal gradually decreased toward the incisal direction. Differing from the mRNA localization, ferritin protein was immunologically undetectable in presecretory or secretory ameloblasts but was found in ameloblasts at the maturation stage, into which iron is known to be incorporated from the bloodstream. Thus, the expression of ferritin mRNA precedes the protein expression in the developmental stages of rat incisor ameloblasts, and the translation of ferritin and its half-life are probably controlled by the iron entry, as has been reported for other cell types.
ISSN:0003-9969
1879-1506
DOI:10.1016/S0003-9969(98)00014-4