Transfer of second messengers through gap junction connexin 43 channels reconstituted in liposomes

Gap junction channels reconstituted in liposomes provide a pathway for the transfer of second messengers. Gap junction channels were formed in the artificial unilamellar liposomes using immunoaffinity-purified connexin 43 gap junction protein from rat brain. Sucrose-permeable and -impermeable liposo...

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Bibliographic Details
Published in:Biochimica et biophysica acta 1998-07, Vol.1372 (2), p.384-388
Main Authors: Kam, Yoonseok, Kim, Doo Yeon, Koo, Soo Kyung, Joe, Cheol O.
Format: Article
Language:English
Subjects:
Animals
Brain Chemistry
Cell communication
Cell Membrane Permeability
Connexin 43
Connexin 43 - metabolism
Cyclic AMP - metabolism
Gap junction
Gap Junctions - metabolism
Inosine Triphosphate - metabolism
Liposome
Liposomes - metabolism
Rats
Rats, Sprague-Dawley
Second messenger
Second Messenger Systems
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Summary:Gap junction channels reconstituted in liposomes provide a pathway for the transfer of second messengers. Gap junction channels were formed in the artificial unilamellar liposomes using immunoaffinity-purified connexin 43 gap junction protein from rat brain. Sucrose-permeable and -impermeable liposomes were separated on the basis of sucrose permeability in the iso-osmolar sucrose density gradient. The liposomes permeable to sucrose were also permeable to a communicating dye molecule, Lucifer yellow. In the present study, we examined the transfer of second messengers through the connexin 43 channels reconstituted in liposomes and first report the direct evidence that the gap junction channels are permeable to second messengers including adenosine 3′,5′-cyclic phosphate and inositol 1,4,5-trisphosphate.
ISSN:0005-2736
0006-3002
1879-2642
DOI:10.1016/S0005-2736(98)00075-3