PDGF-BB enhances expression of, and reduces adhesion to, laminin-5 in vascular smooth muscle cells

The laminin family of extracellular matrix (ECM) proteins plays crucial roles in regulating cellular growth, migration, and differentiation. We report here that laminin-5 is expressed in the tunica media of the rat aorta and pulmonary arteries. Using indirect immunofluorescence microscopy, Western b...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2002-06, Vol.294 (5), p.1017-1022
Main Authors: Kingsley, K, Rust, W.L, Huff, J.L, Smith, R.C, Plopper, G.E
Format: Article
Language:English
Subjects:
Adhesion
Animals
Aorta - cytology
Becaplermin
Cell Adhesion
Cell Adhesion Molecules - biosynthesis
Cell Adhesion Molecules - genetics
Cell Adhesion Molecules - pharmacology
Cells, Cultured
Drug Synergism
Extracellular matrix
Extracellular Matrix Proteins - pharmacology
Growth Substances - pharmacology
Immunohistochemistry
In Situ Hybridization
Integrin
Kalinin
Muscle, Smooth, Vascular - drug effects
Muscle, Smooth, Vascular - metabolism
Muscle, Smooth, Vascular - physiology
PDGF
Platelet-Derived Growth Factor - pharmacology
Proto-Oncogene Proteins c-sis
Rats
Restenosis
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
Transcriptional Activation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The laminin family of extracellular matrix (ECM) proteins plays crucial roles in regulating cellular growth, migration, and differentiation. We report here that laminin-5 is expressed in the tunica media of the rat aorta and pulmonary arteries. Using indirect immunofluorescence microscopy, Western blots, and RT-PCR analysis, we found that primary cultures of rat arterial smooth muscle cells express laminin-5 and deposit it into their insoluble ECM. These cells also attach strongly to laminin-5 via β1 integrin receptors in 30 min adhesion assays. Laminin-5 expression in these cells is upregulated by growth factors in vitro and platelet-derived growth factor (PDGF-BB) stimulation reduces adhesion to laminin-5. As laminin-5 promotes enhanced migration of other cell types, the production of and adhesion to laminin-5 by vascular smooth muscle cells may play a role in the pathological growth and migration of these cells associated with restenosis following vascular injury.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(02)00592-2