Vesicle sizing

A procedure is described which optimizes nonnegative least squares and exponential sampling fitting methods for analysis of dynamic light scattering (DLS) data from aqueous suspensions of vesicle/liposome systems. This approach utilizes a Rayleigh-Gans-Debye form factor for a coated sphere and yield...

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Bibliographic Details
Published in:Biophysical journal 1991-02, Vol.59 (2), p.357-362
Main Authors: Hallett, F.R., Watton, J., Krygsman, P.
Format: Article
Language:English
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Summary:A procedure is described which optimizes nonnegative least squares and exponential sampling fitting methods for analysis of dynamic light scattering (DLS) data from aqueous suspensions of vesicle/liposome systems. This approach utilizes a Rayleigh-Gans-Debye form factor for a coated sphere and yields number distributions which can be compared directly to distributions obtained by freeze-fracture electron microscopy (EM). Excellent agreement between the DLS and EM results are obtained for vesicle size distributions in the 100–200-nm range.
ISSN:0006-3495
1542-0086
DOI:10.1016/S0006-3495(91)82229-9